Transfusion and Apheresis Science
Volume 27, Issue 3 , Pages 217-223, December 2002

Prediction of fetal Rh D and Rh CcEe phenotype from maternal plasma with real-time polymerase chain reaction☆

  • Tobias J Legler

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-551-39-8690; fax: +49-551-39-8691
    • ,
  • Rainer Lynen

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Jens-Holger Maas

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Gerhard Pindur

      Affiliations

    • Department of Clinical Haemostaseology and Transfusion Medicine, University of Saarland, Homburg/Saar, Germany
    • ,
  • Dietrich Kulenkampff

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Anette Suren

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Rüdiger Osmers

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Michael Köhler

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany

Received 29 April 2002; accepted 20 June 2002.

Abstract 

Real-time PCR methods for the detection of RHD and the C, c, and E allele of RHCE were applied for the prediction of fetal Rh phenotype using maternal plasma. In one of 36 samples investigated the DNA extraction failed. When we tested the remaining 35 samples for Rh antigens which were absent on the mother’s red cells, the fetal D-status was correctly determined in 26 of 27 cases (1 false negative). Fetal C was tested correctly in 23 samples, c was true positive in the only c-negative woman and the fetal E-status was correctly determined in 35 cases. In conclusion real-time PCR of maternal plasma is a non-invasive method to determine fetal RH genotype. However, more studies are required for routine applications because the method is not 100% sensitive.

Keywords:  Maternal, Fetal, Genotyping, Rh, Sensitivity

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☆ This study was supported in part by grant LE 1222/1-1 of “Deutsche Forschungsgemeinschaft”. This work is part of the thesis (Habilitation) of T.J.L.

PII: S1473-0502(02)00068-X

Transfusion and Apheresis Science
Volume 27, Issue 3 , Pages 217-223, December 2002

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