Transfusion and Apheresis Science
Volume 27, Issue 3 , Pages 217-223 , December 2002

Prediction of fetal Rh D and Rh CcEe phenotype from maternal plasma with real-time polymerase chain reaction

  • Tobias J Legler

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-551-39-8690; fax: +49-551-39-8691
    • ,
  • Rainer Lynen

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Jens-Holger Maas

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Gerhard Pindur

      Affiliations

    • Department of Clinical Haemostaseology and Transfusion Medicine, University of Saarland, Homburg/Saar, Germany
    • ,
  • Dietrich Kulenkampff

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Anette Suren

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Rüdiger Osmers

      Affiliations

    • Department of Obstetrics and Gynecology, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany
    • ,
  • Michael Köhler

      Affiliations

    • Department of Transfusion Medicine, University of Göttingen, Robert-Koch-Str. 40, 37075 Göttingen, Germany

Received 29 April 2002 ,Accepted 20 June 2002.

References 

  1. Urbaniak SJ, Greiss MA. RhD haemolytic disease of the fetus and the newborn. Blood Rev. 2000;14:44–61
  2. Lo YM. Fetal RhD genotyping from maternal plasma. Ann. Med. 1999;31:308–312
  3. Avent ND, Finning KM, Martin PG, Soothill PW. Prenatal determination of fetal blood group status. Vox Sang. 2000;78(Suppl 2):155–162
  4. Singleton BK, Green CA, Avent ND, Martin PG, Smart E, Daka A, et al.  The presence of an RHD pseudogene containing a 37 base pair duplication and a nonsense mutation in Africans with the Rh D-negative blood group phenotype. Blood. 2000;95:12–18
  5. Shao CP, Maas JH, Su YQ, Köhler M, Legler TJ. Molecular background of Rh D-positive, D-negative, Del and weak D phenotypes in Chinese. Vox Sang, in press
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  8. Rijnders RJ, van der Schoot CE, Bossers B, de Vroede MA, Christiaens GC. Fetal sex determination from maternal plasma in pregnancies at risk for congenital adrenal hyperplasia. Obstet. Gynecol. 2001;98:374–378
  9. Lo YM, Hjelm NM, Fidler C, Sargent IL, Murphy MF, Chamberlain PF, et al.  Prenatal diagnosis of fetal RhD status by molecular analysis of maternal plasma. N. Engl. J. Med. 1998;339:1734–1738
  10. Faas BH, Beuling EA, Christiaens GC, von dem Borne AE, van der Schoot CE. Detection of fetal RHD-specific sequences in maternal plasma [letter]. Lancet. 1998;352:1196
  11. Nelson M, Eagle C, Langshaw M, Popp H, Kronenberg H. Genotyping fetal DNA by non-invasive means: extraction from maternal plasma. Vox Sang. 2001;80:112–116
  12. Zhang J, Fidler C, Murphy MF, Chamberlain PF, Sargent IL, Redman C, et al.  Determination of fetal RhD status by maternal plasma DNA analysis. Ann. N. Y. Acad. Sci. 2000;906:153–155
  13. Ariga H, Ohto H, Busch MP, Imamura S, Watson R, Reed W, et al.  Kinetics of fetal cellular and cell-free DNA in the maternal circulation during and after pregnancy: implications for noninvasive prenatal diagnosis. Transfusion. 2001;41:1524–1530
  14. Heermann, Klaus-Hinrich. Magnetic pin for concentrating and separating particles. German Patent DE 197 30 497 C2, international Patents pending

☆ This study was supported in part by grant LE 1222/1-1 of “Deutsche Forschungsgemeinschaft”. This work is part of the thesis (Habilitation) of T.J.L.

PII: S1473-0502(02)00068-X

Transfusion and Apheresis Science
Volume 27, Issue 3 , Pages 217-223 , December 2002

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