Transfusion and Apheresis Science
Volume 28, Issue 1 , Pages 35-42 , February 2003

Follow-up of the T-cell clonality in Sezary patients treated by extracorporeal photopheresis using a new assay: the immunoscope technique

  • Saskia Ingen-Housz-Oro

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • Département d’Immunologie, Unitéde Biologie Moléculaire du Gène, Institut Pasteur, INSERM U277, 25 rue du Dr. Roux, Paris 75015, France
    • Corresponding Author InformationCorresponding author. Address: Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Annette Bussel

      Affiliations

    • Unité de thérapie cellulaire et de clinique transfusionnelle, Hôpital Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Béatrice Flageul

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Louis Dubertret

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Laurence Michel

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Gabriel Gachelin

      Affiliations

    • Département d’Immunologie, Unitéde Biologie Moléculaire du Gène, Institut Pasteur, INSERM U277, 25 rue du Dr. Roux, Paris 75015, France
    • ,
  • Philippe Kourilsky

      Affiliations

    • Département d’Immunologie, Unitéde Biologie Moléculaire du Gène, Institut Pasteur, INSERM U277, 25 rue du Dr. Roux, Paris 75015, France
    • ,
  • Hervé Bachelez

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • ,
  • Philippe Musette

      Affiliations

    • Institut de recherche sur la peau, INSERM U532, Hôpital, Saint-Louis, Paris 75475, Cedex 10, France
    • Département d’Immunologie, Unitéde Biologie Moléculaire du Gène, Institut Pasteur, INSERM U277, 25 rue du Dr. Roux, Paris 75015, France

References 

  1. Edelson R, Berger C, Gasparro F, et al.  Treatment of cutaneous T-cell lymphoma by extracorporeal photochemotherapy. N. Engl. J. Med. 1987;316:297–303
  2. Fraser-Andrews E, Seed P, Whittaker S, et al.  Extracorporeal photopheresis in Sezary syndrome. No significant effect in the survival of 44 patients with a peripheral blood T-cell clone. Arch. Dermatol. 1998;134:1001–1005
  3. Gottlieb SL, Wolfe JT, Fox FE, et al.  Treatment of cutaneous T-cell lymphoma with extracorporeal photopheresis monotherapy and in combination with recombinant interferon alfa: a 10 years experience at a single institution. J. Am. Acad. Dermatol. 1996;35:946–957
  4. Dippel E, Schrag H, Goerdt D, et al.  Extracorporeal photopheresis and interferon-alfa in advanced cutaneous T-cell lymphoma. Lancet. 1997;350:32–33
  5. Rook AH, Prystowsky MB, Cassin M, et al.  Combined therapy for Sezary syndrome with extracorporeal photochemotherapy and low-dose interferon-alfa therapy. Clinical, molecular, and immunologic observations. Arch. Dermatol. 1991;127:1535–1540
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  10. Witzens M, Mohler T, Willhauck M, et al.  Detection of clonaly rearrenged T-cell receptor gamma chain genes from T-cell malignancies and acute inflammatory rheumatic disease using PCR amplification, PAGE, and automated analysis. Ann. Hematol. 1997;74:123–130
  11. Guitard J, Kaul K. A new polymerase chain reaction based method for detection of T-cell clonality in patients with possible cutaneous T-cell lymphoma. Arch. Dermatol. 1999;135:158–162
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  14. Wolfe J, Chooback L, Finn D, et al.  Large transformation following detection of minimal residual disease in cutaneous T-cell lymphoma: molecular and in situ analysis of a single neoplastic T-cell clone expressing the identical T-cell receptor. J. Clin. Oncol. 1995;13:1751–1757
  15. Pannetier C, Levraud JP, Lim A, et al.  The Immunoscope approach for the analysis of T cell repertoires. In:  Oksenberg JR editors. The antigen T-cell receptor: selected protocols and applications. London: Chapman and Hall; 1998;p. 287–325
  16. Musette P, Even J, Dubertret L, et al.  In:  Nickoloff B editors. The immunoscope analysis of T lymphocytes infiltrating melanocytic tumors. Ann Arbor, MI: Humanae Press; 2001;
  17. Bour H, Puisieux I, Even J, et al.  T-cell repertoire analysis in chronic plaque psoriasis suggests an antigen-specific immune response. Human Immunol. 1999;60:665–676
  18. Mempel M, Flageul B, Suarez F, et al.  Comparaison of the T-cell patterns in leprous and cutaneous sarcoid granulomas: presence of Valfa24-invariant NKT cells in T-cell reactive leprosy together with highly biased TCR Valfa repertoire. Am. J. Pathol. 2000;157:509–523
  19. Andreu G, Leon A, Heshmati F, et al.  Extracorporeal photochemotherapy: evaluation of two techniques and use in connective tissue disorders. Transfus. Sci. 1994;15:443–454
  20. Jackow CM, Cather JC, Hearne V, et al.  Association of erythrodermic cutaneous T-cell lymphoma, superantigen-positive staphylococcus aureus, and oligoclonal T-cell receptor Vb gene expansion. Blood. 1997;89:32–40
  21. Gilks CB, Ho VC, Gascoyne RD, et al.  T-cell receptor variable region gene expression in cutaneous T-cell lymphoma. J. Cutan. Pathol. 1992;19:21–26
  22. Jack AS, Boylston AW, Carrel S, et al.  Cutaneous T-cell lymphoma cells employ a restricted range of T-cell antigen receptor variable region receptor. Am. J. Pathol. 1990;136:17–21
  23. Musette P, Becquet D, Delarbre C, et al.  Expansion of a recurrent Vβ5.3+T cell population in newly diagnosed and untreated HLA-DR2 multiple sclerosis patient. Proc. Natl. Acad. Sci. U.S.A. 1996;93:12461–12466
  24. Lim A, Baron V, Ferradini L, et al.  Combination of MHC-peptide multimer based T cell sorting with the Immunoscope permits sensitive ex vivo quantitation and follow-up of human CD8+T cell immune response. J. Immunol. Methods. 2002;261:177–194
  25. Mc Henry PM, Campbell I, Mackie RM. Assessment of an anti-T-cell receptor variable region antibody panel in cutaneous T-cell lymphoma. Br. J. Dermatol. 1994;130:595–598
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  27. Weinberg JM, Jaworsky C, Benoit BM, et al.  The clonal nature of Sezary cells. Blood. 1995;86:4257–4262
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PII: S1473-0502(02)00098-8

doi: 10.1016/S1473-0502(02)00098-8

Transfusion and Apheresis Science
Volume 28, Issue 1 , Pages 35-42 , February 2003

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