Transfusion and Apheresis Science
Volume 28, Issue 1 , Pages 43-50, February 2003

PUVA apoptotic response in activated and resting human lymphocytes

  • A.E Heng

      Affiliations

    • Service de Néphrologie (Pr Deteix) Henri Dunant BP69 63003, CHU Clermont-Ferrand Cedex, France
    • Corresponding Author InformationCorresponding author. Tel.: +33-4-73-75-14-25; fax: +33-4-73-75-11-83
    • ,
  • B Sauvezie

      Affiliations

    • Unité d’Immunologie Clinique et laboratoire d’Immunologie B (Pr B Sauvezie), 63003 CHU Clermont-Ferrand Cedex, France
    • ,
  • L Genestier

      Affiliations

    • INSERM U503 (Pr JP Revillard)CHU, 69000, HCL Lyon, France
    • ,
  • F Demeocq

      Affiliations

    • Unité Bioclinique (Pr F Demeocq), Departement de pédiatrie, 63003 CHU Clermont-Ferrand Cedex, France
    • ,
  • A Dosgilbert

      Affiliations

    • Unité d’Immunologie Clinique et laboratoire d’Immunologie B (Pr B Sauvezie), 63003 CHU Clermont-Ferrand Cedex, France
    • ,
  • P Deteix

      Affiliations

    • Service de Néphrologie (Pr Deteix) Henri Dunant BP69 63003, CHU Clermont-Ferrand Cedex, France

Abstract 

In extracorporeal photopheresis (ECP) collected cells are treated by 8 methoxypsoralen and UVA (PUVA) which induced apoptosis. The mechanism of action of reinfused cell is unclear. A vaccination model postulates an efficient presentation of apoptotic alloreactive cells to the patient immune system. The efficiency may depend upon a predominence of apoptotic alloreactive cells after PUVA. Such selectivity could result from their activation. We studied apoptosis in resting and PHA-activated lymphocytes. Both were equally susceptible. Changes in early apoptosis were possibily missed. We evaluated the effect of preincubation before PUVA. During preincubation monocyte could affect lymphocytes susceptibility to apoptosis as an increase of number of apoptotic cells was observed after 72 hours in stimulated and resting cells.

Our findings do not preclude a selectivity of other PUVA effects since expression of membrane marker also targets to PUVA is modified by activation.

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PII: S1473-0502(02)00099-X

doi:10.1016/S1473-0502(02)00099-X

Transfusion and Apheresis Science
Volume 28, Issue 1 , Pages 43-50, February 2003

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