Transfusion and Apheresis Science
Volume 30, Issue 1 , Pages 23-28 , February 2004

Blood component fractionation: manual versus automatic procedures

  • Daniela Pasqualetti

      Affiliations

    • Department of Cell Biotechnology and Hematology, University “La Sapienza”, Blood Bank, Via Chieti 7, Rome 00161, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39-06-85795-524; fax: +39-06-85795-501
  • ,
  • Alessandro Ghirardini

      Affiliations

    • Istituto Superiore di Sanità, Laboratory of Epidemiology and Biostatistics, Rome, Italy
  • ,
  • Maria Cristina Arista

      Affiliations

    • Department of Cell Biotechnology and Hematology, University “La Sapienza”, Blood Bank, Via Chieti 7, Rome 00161, Italy
  • ,
  • Stefania Vaglio

      Affiliations

    • Department of Cell Biotechnology and Hematology, University “La Sapienza”, Blood Bank, Via Chieti 7, Rome 00161, Italy
  • ,
  • Azis Fakeri

      Affiliations

    • Department of Cell Biotechnology and Hematology, University “La Sapienza”, Blood Bank, Via Chieti 7, Rome 00161, Italy
  • ,
  • Alan A Waldman

      Affiliations

    • Waldman Biomedical Consultancy, Oceaside, New York, USA
  • ,
  • Gabriella Girelli

      Affiliations

    • Department of Cell Biotechnology and Hematology, University “La Sapienza”, Blood Bank, Via Chieti 7, Rome 00161, Italy

References 

  1. Guide to the preparation, use and quality assurance of blood components. 6th ed.. Strasbourg: Council of Europe Publishing; 2000;
  2. The American Association of Blood Banks Annual Report 1990. Arlington, Va, American Association of Blood Banks, 1990
  3. Prins HK, de Bruijn JCGH, Henrichs HPJ, Loos JA. Prevention of microaggregate formation by removal of “buffycoats”. Vox Sang. 1980;39:48–51
  4. Fijnheer R, Veldman HA, van der Eertwegh AJ, Gouwerok CW, Homburg CH, Boomgaard MN, et al.  In vitro evaluation of buffy-coat-derived platelet concentrates stored in a synthetic medium. Vox Sang. 1991;60:16–22
  5. Hogman CF, Eriksson L, Hedlund K, Wallvik J. The bottom and top system: a new technique for blood component preparation and storage. Vox Sang. 1988;55:211–217
  6. Fijnheer R, Pieters RNI, de Korte D, Gouwerok CWN, Dekker WJA, Reesink HW, et al.  Platelet activation during preparation of platelet concentrates: a comparison of the platelet-rich plasma and the buffy coat methods. Transfusion. 1990;30:634–638
  7. Lozano M, Estebanell E, Cid J, Diaz-Ricart M, Mazzara R, Ordinas A, et al.  Platelet concentrates prepared and stored under currently optimal conditions: minor impact on platelet adhesive and cohesive functions after storage. Transfusion. 1999;39:951–959
  8. Anderson KC, Ness PM. Scientific basis of Transfusion medicine. 2nd ed.. Implications for clinical basis. Saunders Company; 2000;
  9. Kretschmer V, Tesche U, Richter P. Improvement of the separation of blood by modification of separators Optipress and Biotrans Separator. Beitr. Infus. 1992;30:112–121
  10. Hurtado C, Bonanad S, Soler MA, Mirabet V, Blasco I, Planelles MD, et al.  Quality analysis of blood components obtained by automated buffy-coat layer removal with a Top & Bottom system (Optipress II). Haematologica. 2000;85:390–395
  11. van Rhenen DJ, Vermeij J, de Voogt J, Bernes JC, Payrat JM. Quality and standardization in blood component preparation with an automated blood processing technique. Transfus. Med. 1998;8:319–324
  12. Dzik S, Moroff G, Dumont L  for the BEST Working Party of the ISBT . A multicenter study evaluating three methods for counting residual WBCs in WCB-reduced blood components: nageotte hemocytometry, flow cytometry, and microfluorometry. Transfusion. 2000;40:513–520
  13. Wallace EL, Churchill WH, Surgenor DM, Cho GS, McGurk S. Collection and transfusion of blood and blood components in the United States, 1994. Transfusion. 1998;38:625–636
  14. The Trial to reduce Alloimmunization to Platelet Study Group . Leukocyte reduction and ultraviolet B irradiation of platelets to prevent alloimmunization and refratoriness to platelet transfusions. N. Engl. J. Med. 1997;25:1861–1869
  15. Kretschmer V, Bierann E, Loh H. Separation of platelet concentrates (PC) from buffy-coats (BC) using the bottom and top (BAT) drainage system. Transfus. Sci. 1990;11:363–366
  16. Rebulla P, Bertolini F, Porretti L, Marangoni F, Smacchia C, Marconi M, et al.  Platelet concentrates from buffy coats: improved conditions for preparation and evaluation in routine clinical use. Transfus. Sci. 1993;14:41–46
  17. Kostelijk EH, Klomp AJA, Engbers GHM, Gouwerok CWN, Verhoeven AJ, van Aken WG, et al.  Improved platelet compatibility of water vapour glow discharge treated non woven poly(ethylene terephthalate)leukocyte-reduction filters for different types of platelet concentrates. Transfus. Med. 2001;11:199–205

PII: S1473-0502(03)00201-5

doi: 10.1016/j.transci.2003.07.002

Transfusion and Apheresis Science
Volume 30, Issue 1 , Pages 23-28 , February 2004