Transfusion and Apheresis Science
Volume 35, Issue 1 , Pages 33-41 , August 2006

In vitro testing of platelets using the thromboelastogram, platelet function analyzer, and the clot signature analyzer to predict the bleeding time

Received 6 December 2005 ,Accepted 13 February 2006.

References 

  1. Murphy S, Rebulla P, Bertolini F, et al. In vitro assessment of the quality of stored platelet concentrates. The BEST (Biomedical Excellence for Safer Transfusion) Task Force of the International Society of Blood Transfusion. Transfus Med Rev. 1994;8:29–36
  2. Valeri CR, Feingold H, Marchionni LD. The relation between response to hypotonic stress and the 51Cr recovery in vivo of preserved platelets. Transfusion. 1974;4:331–337
  3. Khuri SF, Healey N, MacGregor H, Barnard MR, Szymanski IO, Birjiniuk V, et al. Comparison of the effects of transfusions of cryopreserved and liquid-preserved platelets on hemostasis and blood loss after cardiopulmonary bypass. J Thorac Cardiovasc Surg. 1999;117:172–184
  4. Barnard MR, MacGregor H, Ragno G, Pivacek LE, Khuri SF, Michelson AD, et al. Fresh, liquid-preserved, and cryopreserved platelets: adhesive surface receptors and membrane procoagulant activity. Transfusion. 1999;39:880–888
  5. International Forum . Evaluation of stored platelets. Vox Sang. 2004;86:203–223
  6. Harker LA, Slichter SJ. The bleeding time as a screening test for the evaluation of platelet function. N Engl J Med. 1972;287:155–159
  7. Gerrard JM, Docherty JC, Israels SJ, et al. A reassessment of the bleeding time: association of age, hematocrit, platelet function, von Willebrand factor, and bleeding time thromboxane B2 with the length of the bleeding time. Clin Invest Med. 1989;12:165–171
  8. Valeri CR, MacGregor H, Cassidy G, Tinney R, Pompei F. Effects of temperature on bleeding time and clotting time in normal male and female volunteers. Crit Care Med. 1995;23:698–704
  9. Crowley JP, Metzger JB, Valeri CR. The volume of blood shed during the bleeding time correlates with the peripheral venous hematocrit. Am J Clin Path. 1997;108:579–584
  10. Valeri CR, Crowley JP, Loscalzo J. The red cell transfusion trigger: has a sin of commission now become a sin of omission?. Transfusion. 1998;38:602–610
  11. Chandler WL. The thromboelastograph and the thromboelastograph technique. Semin Thromb Hemost. 1995;21:1–6
  12. Traverso CI, Caprini JA, Arcelus J, Arcelus IM. Thromboelastographic modifications induced by intra-venous and subcutaneous heparin administration. Semin Thromb Hemost. 1995;21:53–58
  13. Summaria L. Thromboelastographic study of fibrinolytic agents. Semin Thromb Hemost. 1995;21:63–71
  14. Valeri CR, Srey R, Tilahun D, Ragno G. The in vitro effects of poly-N-acetylglucosamine on the activation of platelets in platelet-rich plasma with and without red blood cells. J Trauma (Suppl). 2004;57:522–525
  15. Li CKN, Hoffman TJ, Hsieh PY, Malik S, Watson W, Xylum CSA. Automated system for assessing hemostasis in simulated vascular flow. Clin Chem. 1997;43:1788–1790
  16. Carcao MD, Blanchette VS, Dean JA, He L, Kern MA, Stain AM, et al. The platelet function analyzer (PFA-100®): a novel in-vitro system for evaluation of primary haemostasis in children. Br J Haematol. 1998;101:70–73
  17. Rodgers RPC, Levin J. A critical reappraisal of the bleeding time. Semin Thromb Hemost. 1991;16:1–20
  18. Lind SE. The bleeding time does not predict surgical bleeding. Blood. 1991;77:2547–2552
  19. Khuri SF, Wolfe JA, Josa M, Axford TC, Szymanski IO, Assousa SN, et al. Hematologic changes during and after cardiopulmonary bypass and their relationship to the bleeding time and nonsurgical blood loss. J Thorac Cardiovasc Surg. 1992;104:94–107
  20. Khuri SF, Valeri CR, Treanor P. Bleeding during and after cardiopulmonary bypass: a 15-year institutional experience. In:  Pifarre R editors. Management of bleeding in cardiovascular surgery. Philadelphia: Hanley & Belfus; 2000;p. 145–160[Chapter 8]
  21. Babson SR, Babson AL. Development and evaluation of a disposable device for performing simultaneous duplicate bleeding time determinations. Am J Clin Pathol. 1978;70:406–408
  22. Valeri CR, Feingold H, Cassidy G, Ragno G, Khuri SF, Altschule MD. Hypothermia-induced reversible platelet dysfunction. Ann Surg. 1987;205:175–181
  23. Valeri CR, Cassidy G, Pivacek LE, Ragno G, Lieberthal W, Crowley JP, et al. Anemia-induced increase in the bleeding time: implications for treatment of non-surgical blood loss. Transfusion. 2001;41:977–983

 This work was supported by the US Navy (Office of Naval Research Contract N00014-94-C-0149) and by funding provided to the US Navy Bureau of Medicine and Surgery.

PII: S1473-0502(06)00088-7

doi: 10.1016/j.transci.2006.02.003

Transfusion and Apheresis Science
Volume 35, Issue 1 , Pages 33-41 , August 2006