Transfusion and Apheresis Science
Volume 42, Issue 1 , Pages 11-19 , February 2010

CFSE flow cytometric quantification of lymphocytic proliferation in extracorporeal photopheresis: Use for quality control

  • Bertrand Evrard

      Affiliations

    • CHU Clermont-Ferrand, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • Univ Clermont1, Faculty of Medicine-Pharmacy, F-63003 Clermont-Ferrand, France
    • Corresponding Author InformationCorresponding author. Address: Department of Immunology, Hotel-Dieu, Boulevard Leon Malfreyt, F-63058 Clermont-Ferrand, France. Tel.: +33 4 73 750 662; fax: +33 4 73 750 637.
    • ,
  • Annie Dosgilbert

      Affiliations

    • CHU Clermont-Ferrand, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • ,
  • Nathalie Jacquemot

      Affiliations

    • Univ Clermont1, Faculty of Medicine-Pharmacy, F-63003 Clermont-Ferrand, France
    • ,
  • François Demeocq

      Affiliations

    • CHU Clermont-Ferrand, Department of Pediatrics, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • ,
  • Thibault Gilles

      Affiliations

    • Univ Clermont1, Faculty of Medicine-Pharmacy, F-63003 Clermont-Ferrand, France
    • ,
  • Jacques Chassagne

      Affiliations

    • Centre Jean Perrin, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • ,
  • Marc Berger

      Affiliations

    • CHU Clermont-Ferrand, Department of Hematology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • ,
  • Arlette Tridon

      Affiliations

    • CHU Clermont-Ferrand, Department of Immunology, Hotel-Dieu Hospital, F-63058 Clermont-Ferrand, France
    • Univ Clermont1, Faculty of Medicine-Pharmacy, F-63003 Clermont-Ferrand, France

Received 28 July 2008 ,Accepted 18 September 2009.

References 

  1. Edelson R, Berger C, Gasparro F, Jegasothy B, Heald P, Wintroub B, et al. Treatment of cutaneous T-cell lymphoma by extracorporeal photochemotherapy. Preliminary results. N Engl J Med. 1987;316:297–303
  2. McKenna KE, Whittaker S, Rhodes LE, Taylor P, Lloyd J, Ibbotson S, et al. Evidence-based practice of photopheresis 1987–2001: a report of a workshop of the British Photodermatology Group and the U.K. Skin Lymphoma Group. Br J Dermatol. 2006;154:7–20
  3. Kanold J, Merlin E, Halle P, Paillard C, Marabelle A, Rapatel C, et al. Photopheresis in pediatric graft-versus-host disease after allogeneic marrow transplantation: clinical practice guidelines based on field experience and review of the literature. Transfusion. 2007;47:2276–2289
  4. Schooneman F. Extracorporeal photopheresis technical aspects. Transfus Apher Sci. 2003;28:51–61
  5. Jacob MC, Manches O, Drillat P, Richard MJ, Plumas J, Chaperot L, et al. Quality control for the validation of extracorporeal photopheresis process using the Vilbert–Lourmat UV-A irradiation’s system. Transfus Apher Sci. 2003;28:63–70
  6. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods. 1994;171:131–137
  7. Lyons AB. Divided we stand: tracking cell proliferation with carboxyfluorescein diacetate succinimidyl ester. Immunol Cell Biol. 1999;77:509–515
  8. Lyons AB. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J Immunol Methods. 2000;243:147–154
  9. Angulo R, Fulcher DA. Measurement of Candida-specific blastogenesis: comparison of carboxyfluorescein succinimidyl ester labelling of T cells, thymidine incorporation, and CD69 expression. Cytometry. 1998;34:143–151
  10. Fulcher D, Wong S. Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory. Immunol Cell Biol. 1999;77:559–564
  11. Plumas J, Drillat P, Jacob MC, Richard MJ, Favrot MC. La photochimiothérapie dans le traitement de pathologies du système lymphocytaire T. Bull Cancer. 2003;90:763–770
  12. Aubin F, Salard D, Pouthier F, Hervé P, Humbert P. Méd/Sci. 1999;15:983–989
  13. Marin L, Minguela A, Torio A, Moya-Quiles MR, Muro M, Montes-Ares O, et al. Flow cytometric quantification of apoptosis and proliferation in mixed lymphocyte culture. Cytometry A. 2003;51:107–118
  14. Chen JC, Chang ML, Muench MO. A kinetic study of the murine mixed lymphocyte reaction by 5,6-carboxyfluorescein diacetate succinimidyl ester labeling. J Immunol Methods. 2003;279:123–133
  15. Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, et al. Low incidence of acute rejection after living-donor liver transplantation: immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique. Transplantation. 2005;79:1262–1267
  16. Heshmati F. Mechanisms of action of extracorporeal photochemotherapy. Transfus Apher Sci. 2003;29:61–70
  17. Marshall SR. Technology insight: ECP for the treatment of GvHD – can we offer selective immune control without generalized immunosuppression?. Nat Clin Pract Oncol. 2006;3:302–314

PII: S1473-0502(09)00170-0

doi: 10.1016/j.transci.2009.10.002

Transfusion and Apheresis Science
Volume 42, Issue 1 , Pages 11-19 , February 2010

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