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Abstract
The neutrophil is a short-lived cell because it has limited biosynthetic capacity
to repair and maintain its component parts. Studies on neutrophil storage show generalized
defects involving energy metabolism, phagocytosis, microbial killing, chemotaxis,
membrane receptors, microtubules, microfilaments, and stimulus—response coupling.
Some storage conditions are more detrimental to certain functions than others, e.g.
cold induces a severe chemotactic defect. Thus, room temperature storage is preferable
to refrigerated storage. For optimal storage, it is necessary to have sufficient glucose
in the medium and to maintain an adequate pH. During storage, a dismantling of the
functional integrity of the cell occurs with the most highly integrated functions
being lost initially, while primitive functions are retained the longest. With minor
exceptions, the generalized metabolic, structural, and functional defects noted in
stored neutrophils are not peculiar to storage but most likely mirror the inexorable
in vivo programmed senescence of the neutrophil.
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© 1988 Published by Elsevier Inc.
