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Abstract
Leukodepleted or leukocyte-poor blood products (fresh-frozen plasma, packed red cell
and platelet concentrates in particular) are widely used in current clinical practice.
However, because the monitoring of leukodepletion efficiency is generally carried
out (if at all) using the labour-intensive and relatively inaccurate manual Nageotte
chamber technique, it is clear that any increased demand for leukodepletion monitoring
would be difficult, if not impossible, to meet. As the need to identify an automated
alternative to the Nageotte technique is important, this study was undertaken to evaluate
such a possibility. White blood cells were enumerated in a representative series of
filtered and non-filtered human blood components by both microscopic counting in the
Nageotte chamber, and with the Abbott CD3500 automated haematology analyser. For the
Nageotte estimate, a single analysis was made in accordance with standard procedures,
whereas the automated analysis was achieved by making six replicate counts and determining
the mean of four replicates after excluding the highest and lowest estimates. To determine
linearity limits of the manual and automated procedures, freshly isolated leukocytes
were admixed with cell-free plasmapheresis plasma. Reasonable reproducibility (mean
CV 10% for cell counts exceeding 100 cells/μL) and good linearity (r > 0.9) were observed for CD3500 determinations in four separate experiments. The
manual and automated measurements also correlated well (r > 0.9) with no obvious inter-method bias for cell counts up to 40 cells/μL although
there was some suggestion of lower absolute CD3500 counts in the range 40–130 cells/μL.
For the comparative studies with filtered and non-filtered blood products, no significant
method bias was seen with 70 individual red cell concentrates, but systematically
higher CD3500 white blood cell counts were observed in the series of 68 platelet concentrates
(probably due to the presence of platelet clumps). This study concludes that automation
of white cell counts in blood products with the CD3500 analyser is feasible for quality
control in the preparation of fresh-frozen plasma and red cell concentrates but is
limited for the analysis of filtered platelet concentrates.
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Article info
Publication history
Accepted:
May 5,
1997
Received:
February 20,
1997
Identification
Copyright
© 1997 Published by Elsevier Inc.
