Submitted paper| Volume 18, ISSUE 4, P495-503, December 1997

In-process cell counts during preparation of platelet concentrates from buffy coats

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      To ensure the quality of platelet concentrates (PCs), we studied in-process recoveries of blood cell counts in pooled PCs derived from four or five buffy coats (BCs) from Biopack Compoflex Systems in Bern (PC-BC/4 or PC-BC/5) and from five BCs from Optipac (Baxter) in Zurich (PC-BC/5). BCs were pooled employing a sterile connecting device and flushing them with 300 mL of platelet additive solution. The pools were centrifuged for 12 min at 500g at 20°C and filtered with PALL's Auto-Stops™ BC-leukocyte removal filter. Automated platelet counting was performed on whole blood donation, on single BC, on pooled BC and in the final product. Four out of 10 PC-BC/4 (=40%) and 29 out of 30 PC-BC/5 (=97%) had a total platelet count of >200 × 109 platelets. Average percentage recoveries in PC compared to the pre-centrifugation BC pools were similar with the Biopack Compoflex and the Optipac systems, 62% and 57% respectively, whereby the absolute platelet count per one donation was similar, i.e. 49.5×109, 55×109 and 53 × 109 in PC-BC/4 and PC-BC/5 from Bern and PC-BC/5 from Zurich. There was a significant positive correlation between the inital number of BCs taken for pooling and the final platelet counts in the PCs. In order to recover a minimal platelet content of 200 × 109 platelets per pooled unit, it is safer to start out with five rather than with four donations unless recoveries during the production steps can be improved.
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