Research Article| Volume 20, ISSUE 1, P21-27, February 1999

Peripheral blood stem cell collection and transplantation using the Haemonetics Multi Component System


      Aim: To assess clinical usefulness of an intermittent-flow blood cell separator in peripheral blood stem cell (PBSC) collection and transplantation. Results: the Haemonetics Multi Component System (Multi) was used to collect PBSC (52 aphereses in 17 patients). The mean processing blood volume and the mean PBSC yield were 7407 ml and 2.16 × 106 CD34+ cells/kg, respectively. When CD34+ cells exceeded 0.3% of the peripheral WBC, more than 2.0 × 106 CD34+ cells/kg could be collected by a single apheresis. Eight patients underwent PBSC transplantation after high-dose chemotherapy. Hematopoietic recovery was achieved in a median period of 10 days. Conclusions: (1) A single-arm, light-weight machine has sufficient capability to collect PBSC. (2) The percentage of CD34+ cells in the peripheral WBC is a good predictor of the CD34+ cell yield of the collection.
      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'


      Subscribe to Transfusion and Apheresis Science
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect


      1. Kessinger A, Armitage JO, Smith DM, Landmark JD, Bierman PJ, Weisenburger DD. High-dose therapy and autologous peripheral blood stem cell transplantation for patients with lymphoma. Blood 1989;74:1260–5

      2. To LB, Roberts MM, Haylock DN, Dyson PG, Branford AL, Thorp D, Ho JQ, Dart GW, Horvath N, Davy ML., et al. Comparison of haematological recovery times and supportive care requirements of autologous recovery phase peripheral blood stem cell transplants, autologous bone marrow transplants and allogeneic bone marrow transplants. Bone Marrow Transplant 1992;9:277–84

      3. Norol F, Scotto F, Duedari N, Beaujean F. Peripheral blood stem cell collection with a blood cell separator. Transfusion 1993;33:894–7

      4. Rosenfeld CS, Cullis H, Tarosky T, Nemunaitis J. Peripheral blood stem cell collection using the small volume collection chamber in the Fenwall CS-3000 Plus blood cell separator. Bone Marrow Transplant 1994;13:131–4

      5. To LB, Stemmelin GR, Haylock DN, Bayly JL, Thorp D, Rawling CM, Trimboli S, Juttner CA. Collection efficiency on the Fenwall CS3000 when using filgrastim (recombinant methionyl human granulocyte colony-stimulating factor) as a peripheral blood stem cell mobilization agent. J Clin Apheresis 1994;9:17–20

      6. Pierelli L, Menichella G, Paoloni A, Vittori M, Foddai ML, Serafini R, Rumi C, Mitschulat H, Rossi PL, Scambia G., et al. Evaluation of a novel automated protocol for the collection of peripheral blood stem cells mobilized with chemotherapy or chemotherapy plus G-CSF using the Fresenius AS104 cell separator. J Hematother 1993;2:145–53

      7. Makino S, Harada M, Akashi K, Taniguchi S, Shibuya T, Inaba S, Niho Y. A simplified method for cryopreservation of peripheral blood stem cells at −80°C without rate-controlled freezing. Bone Marrow Transplant 1991;8:239–44

      8. Fauser AA, Messner HA. Granuloerythropoietic colonies in human bone marrow, peripheral blood, and cord blood. Blood 1978;52:1243–8

      9. Bernstein ID, Andrews RG, Zsebo KM: Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor. Blood 1991;77:2316–31

      10. Nakamura T, Hashimoto E, Sasada M, Tsutani H, Kagawa D, Ueda T, Uchida M, Domae N, Ando S, Uchino H. Action mechanism of methyl 6-[3-(2-chloroethyl)-3-nitrosoureido]-6-deoxy-alpha-D-glucopyranoside (MCNU) in leukemic cells. Nippon Ketsueki Gakkai Zasshi 1985;48:734–41

      11. Bender JG, To LB, Williams S, Schwartzberg LS. Defining a therapeutic dose of peripheral blood stem cells. J Hematother 1992;1:329–431

      12. Padley D, Strauss RG, Wieland M, Randels MJ. Concurrent comparison of the Cobe Spectra and Fenwall CS3000 for the collection of peripheral blood mononuclear cells for autologous peripheral stem cell transplantation. J Clin Apheresis 1991;6:77–80

      13. Mehta J, Powles R, Cabral S, Shepherd V, Singhal S, Morilla R, Treleaven J. Comparison of Cobe Spectra and Haemonetics MCS-3P cell separators for peripheral blood stem cell harvesting. Bone Marrow Transplant 1995;16:707–9

      14. Haas R, Mohle R, Fruhauf S, Goldschmidt H, Witt B, Flentje M, Wannenmacher M, Hunstein W. Patient characteristics associated with successful mobilizing and autografting of peripheral blood progenitor cells in malignant lymphoma. Blood 1994;83:3787–94

      15. Schwella N, Siegert W, Beyer J, Rick O, Zingsem J, Eckstein R, Serke S, Huhn D. Autografting with blood progenitor cells: predictive value of preapheresis blood cell counts on progenitor cell harvest and correlation of the reinfused cell dose with hematopoietic reconstitution. Ann Hematol 1995;71:227–34

      16. Bensinger WI, Longin K, Appelbaum F, Rowley S, Weaver C, Lilleby K, Gooley T, Lynch M, Higano T, Klarnet J., et al. Peripheral blood stem cells (PBSCs) collected after recombinant granulocyte colony stimulating factor (rhG-CSF): an analysis of factors correlating with the tempo of engraftment after transplantation. Br J Haematol 1994;87:825–31