Abstract
Background
Wound healing is a dynamic process, involving the recruitment of growth factors, cytokines,
chemokines and cellular populations. Recently, the Cord Blood Platelet Gel (CBPG)
has been applied successfully in wound closure and tissue regeneration. Moreover,
its proper combination with stem cell populations such as Mesenchymal Stromal Cells
(MSCs) may positively improve the wound healing process. Based on the above data,
this study aimed to the evaluation of wound healing capacity of MSCs combined with
CBPG under in vitro conditions.
Methods
Initially, CBPG was developed from Cord Blood Units (CBUs). The determination of wound
healing ability of MSCs was performed using the scratch wound assay. In addition,
the morphological features, immunophenotypical characteristics and differentiation
capacity of MSCs were evaluated.
Results
Scratch wound assay results showed, that CBPG could positively stimulate the MSCs
migration. Moreover, MSCs cultured in presence of CBPG were characterized by elongated
shape and improved stemness properties as it was indicated by flow cytometric analysis
and differentiation process.
Conclusion
These results clearly showed the beneficial effect of CBPG in combination with MSCs
in wound healing. The proper combination of CBPG with stem cells strategy may enhance
the healing process in patients with skin erosions.
Keywords
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Article info
Publication history
Published online: January 27, 2020
Accepted:
January 17,
2020
Received in revised form:
January 2,
2020
Received:
November 15,
2019
Identification
Copyright
© 2020 Elsevier Ltd. All rights reserved.
