Abstract
Background
There is paucity of data related to the prevalence of the rare blood group antigens
amongst South Gujarat blood donor population due to unavailability and high cost of
antisera. Therefore it is difficult to screen donors for such rare antigens by gold
standard haemagglutination assay. The single nucleotide polymorphism (SNPs) of Ina and Inb antigens is the base of the PCR based detection methods that help to detect these
alleles in regular voluntary blood donors.
Materials & methods
Blood samples of 200 unrelated regular voluntary blood donors wee collected. DNA was
extracted using phenol-chloroform method and genotyped for Indian (Ina/IN*01, Inb/IN*02) blood group alleles by Sequence Specific PCR. Ina antigen positivity was confirmed by serology test.
Results
Four donors were found heterozygous for Ina antigen i.e. In (a + b+) by SS-PCR and their Ina positivity were confirmed by in-house polyclonal Anti-Ina reagent. SS-PCR was standardized using known heterozygous sample of a blood donor.
The frequency of Ina antigen (2.0 %) was higher than Caucasians, lower than Iranians and Arabs while comparable
to those reported among Indians of Mumbai city.
Conclusion
In absence or unavailability of antisera particularly for low frequency alleles like
Ina, such PCR based method would be extremely helpful to prepare rare donor registry
by screening blood donors’ at large scale. Red cells of Ina positive donors can be used as in-house reagent red cells for screening and identification
of corresponding antibody.
Keywords
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Article info
Publication history
Published online: November 23, 2021
Accepted:
November 20,
2021
Received in revised form:
November 19,
2021
Received:
February 13,
2021
Identification
Copyright
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