Abstract
Background
Platelet transfusion therapy is widely used to prevent hemorrhage in patients with
thrombocytopenia and platelet disorders. The platelet concentrate (PC) quality is
affected by increased storage time, as reflected in the decreased number of platelets,
morphological changes, and impaired functions. This study aimed to analyze the impact
of 5 days storage on platelets count and the expression of CD63, and Annexin V as
activation markers during PC storage.
Methods
Fifty PCs collected from single donors were tested for platelet count on days 0, 3,
and 5 using a Sysmex blood counter. CD61, CD63, and Annexin V expression was analyzed
by a multicolor Navios flow cytometer.
Results
There was a significant decrease in platelet count during 5 days of storage. There
was a direct relationship between storage time and degree of platelet activation.
CD63 had almost double increased expression on day 5 than day 3. Annexin V showed
significantly increased expression on day 3 with minor differences between days 3
and 5.
Conclusion
According to standard blood bank conditions, PC stored for 5 days showed a degree
of in vitro activation as evidenced by CD63 and Annexin V expression, may lead to reduced therapeutic
efficacy. Flow cytometry monitoring platelet activation in PC offers a better understanding
of the changes during PC storage and may help improve platelet products.
Keywords
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Article info
Publication history
Published online: November 25, 2021
Accepted:
November 22,
2021
Received in revised form:
October 31,
2021
Received:
August 24,
2021
Identification
Copyright
© 2021 Elsevier Ltd. All rights reserved.
