Advertisement

Serology and gene sequence analysis of the B(A) subtype of patients in Hunan

  • Fengxia Liu
    Affiliations
    Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
    Search for articles by this author
  • Lan Gu
    Correspondence
    Correspondence to: Department of Blood Transfusion The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.
    Affiliations
    Department of Blood Transfusion, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
    Search for articles by this author

      Highlights

      • In Hunan, the gene frequency of B(A) was 1/26,000, which was the highest among all research reports in the world.
      • ABO*BA.02 was the most common allele encoding the B(A) phenotype in Hunan.
      • The frequency of B(A) phenotype in Hunan was first reported worldwide.

      Abstract

      Introduction

      B(A) is a relatively rare ABO subtype. The frequency of B(A) in European Caucasians is low, but is higher in China. B(A) is common in northern China, gradually decreasing from north to south. The frequency of B(A) has been researched in northern China, but not in southern China. This study aimed to investigate the serological characteristics and molecular mechanism of the B(A) subtype in Hunan.

      Methods

      The phenotypes of ABO blood group were analyzed by blood group serology for the patients in the Third Xiangya Hospital from 2016 to 2020; Exons 1–7 of the ABO gene were screened by PCR-SSP and sequencing.

      Results

      Nine cases were suspected as belonging to the B(A) subtype by serological tests. The activity of D-galactosyltransferase in these patients' serum was significantly higher than that of group B plasma; meanwhile, there was no activity of Nacetylgalactosyltransferase. The ABO genotype was ABO*B.01/O.01.01 or ABO*B.01/O.01.02, and the gene sequencing results confirmed the phenotype as B(A), whose gene frequence was 1/26,000. Allele ABO*BA.02 (1/38,000) had the highest frequency, followed by ABO*BA.04 (1/77,000).

      Conclusion

      Among the patients in Hunan, the most common allele encoding the B(A) phenotype was ABO*BA.02. The identification of the B(A) blood group required serological methods combined with genotyping.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Transfusion and Apheresis Science
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Yamamoto F.
        • McNeill P.D.
        • Yamamoto M.
        • Hakomori S.
        • Harris T.
        • Judd W.J.
        • et al.
        Molecular genetic analysis of the ABO blood group system: 1. Weak subgroups: A3 and B3 alleles.
        Vox Sang. 1993; 64: 116-119
        • Schenkel-Brunner H.
        Human Blood Groups: Chemical and Biochemical Basis of Antigen Specificity.
        second ed. Spingerwien, New York2000
        • Liu C.L.
        • Miao T.H.
        • Liu Y.Q.
        • Yan F.
        • Liu S.F.
        • Liu K.
        Study on allele typing of B(A) blood group.
        Chin J Blood Transfus. 2012; 25: 429-431
        • Jiao L.X.
        • Zhang J.Y.
        • Chen L.
        • Yu X.L.
        • Han Y.
        • Yang F.
        • et al.
        Gene identification of rare B(A) blood group.
        Transfus Apher Sci. 2017; 56: 855-857
        • Shang H.
        • Wang L.
        • Shen Z.
        National Guide to Clinical Laboratory Procedures.
        fourth ed. People’s Medical Publishing House, Beijing2015
        • Yanlian Liang
        • Yuqing S.U.
        • Yinze Zhang
        • Fan Wu
        • Dacheng Li
        Chin J Exp Hematol. 2017; 25: 231-234
        • Li Y.
        • Ma X.Y.
        Practical Hematology, Immunology.
        first ed. Science Press, Beijing2006
        • Danies G.
        Hunan Blood Groups.
        third ed. Wiley-Blackwell, Oxford. UK2013
        • Cohn C.S.
        • Delaney M.
        • Johnson S.T.
        • Katz L.M.
        Technical Manual.
        twentieth ed. American Association of Blood Banks, Bethesda2020
        • Fung K.M.
        • Grossman B.J.
        • Hillyer C.D.
        • Westhoff C.M.
        Technical Manual.
        eighteenth ed. American Association of Blood Banks, Bethesda2014
        • Guo Z.H.
        • Dong X.
        • Zhu Z.Y.
        • Xi L.
        • Liu D.Z.
        Molecular study on CisAB and B(A) blood group in Chinese individuals.
        . Chin J Med Genet. 2004; 21: 321-324
        • Ma H.M.
        • Shi J.L.
        • Yan B.Z.
        • Yan H.
        Analysis of serological characteristics of B(A) blood group.
        J Clin Hematol. 2021; 34: 112-114
        • Guo Z.H.
        • Xiang D.
        • Zhu Z.Y.
        • Liu X.
        • Chen H.P.
        • Wang J.L.
        • et al.
        Serologic and molecular characterization of the B(A) blood group in the Chinese population.
        Immunohematology. 2007; 23: 69-74
        • Chen Q.
        • Li J.
        • Xiao J.
        • Du L.
        • Li M.
        • Yao G.
        Molecular genetic analysis and structure model of a rare B(A)02 subgroup of the ABO blood group system.
        Transfus Apher Sci. 2014; 51: 203-208
        • Jin S.
        • Cai X.H.
        • Liu X.
        • Wang J.L.
        • Lu Q.
        • Shen W.
        • et al.
        Study on cisAB and B( A) subgroups in Shanghai blood donors.
        Chin J Blood Transfus. 2013; 26: 1198-1201
        • Shan Y.Q.
        • Chong J.H.
        • Du C.H.
        • Xu Y.H.
        • Huang H.T.
        Identification and blood transfusion of rare B(A) blood group.
        Clin Lab. 2021; 67