Abstract
Hematopoietic stem cells (HSCs) which are characterized with CD34+ phenotype, have
a pivotal role in blood cell regeneration. They are located in lowest hypoxic areas
in the bone marrow niches. This microenvironment protects them from DNA damage and
excessive proliferation, whereas the oxygenated area driving cells out of quiescent
state into proliferation. Given the resistance of HSCs to hypoxia, it is reasonable
to imagine that they can survive for some time in the absence of oxygen. Here, we
evaluated CD34, Bax, Bcl-2, Bcl-xl, and p53 genes expression after death. Moreover,
we established the ex-vivo development of HSCs using SCF, FLT3, IL-2, and IL-15 cytokines
in culture system. Our finding indicated that although the most of the dead person's
mononuclear cells were alive and adequately expressed the CD34 on their surfaces at
the first day of isolation, the viability and CD34+/Ki-67 expression declined significantly
after culture process. Taken together, our finding indicated that the viability and
CD34+ expression was acceptable on day 0 and could be used as a novel method for therapeutic
purposes.
Keywords
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Article info
Publication history
Published online: May 03, 2022
Accepted:
May 1,
2022
Received in revised form:
April 29,
2022
Received:
January 20,
2022
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
