Advertisement

Bone marrow CD34 positive cells may be suitable for collection after death

      Abstract

      Hematopoietic stem cells (HSCs) which are characterized with CD34+ phenotype, have a pivotal role in blood cell regeneration. They are located in lowest hypoxic areas in the bone marrow niches. This microenvironment protects them from DNA damage and excessive proliferation, whereas the oxygenated area driving cells out of quiescent state into proliferation. Given the resistance of HSCs to hypoxia, it is reasonable to imagine that they can survive for some time in the absence of oxygen. Here, we evaluated CD34, Bax, Bcl-2, Bcl-xl, and p53 genes expression after death. Moreover, we established the ex-vivo development of HSCs using SCF, FLT3, IL-2, and IL-15 cytokines in culture system. Our finding indicated that although the most of the dead person's mononuclear cells were alive and adequately expressed the CD34 on their surfaces at the first day of isolation, the viability and CD34+/Ki-67 expression declined significantly after culture process. Taken together, our finding indicated that the viability and CD34+ expression was acceptable on day 0 and could be used as a novel method for therapeutic purposes.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Transfusion and Apheresis Science
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Asl K.D.
        • Velaei K.
        • Rafat A.
        • Nasrabadi H.T.
        • Movassaghpour A.A.
        • Mahdavi M.
        • et al.
        The role of KIR positive NK cells in diseases and its importance in clinical intervention.
        Int Immunopharmacol. 2021; 92107361
        • Grant W.C.
        • Root W.S.
        The relation of O2 in bone marrow blood to post-hemorrhagic erythropoiesis.
        Am J Physiol-Legacy Content. 1947; 150: 618-627
        • Cipolleschi M.
        • Olivotto M.
        Hemopoietic progenitor cells are sensitive to the cytostatic effect of pyruvate.
        Exp Hematol. 1987; 15: 137-142
        • Eliasson P.
        • Rehn M.
        • Hammar P.
        • Larsson P.
        • Sirenko O.
        • Flippin L.A.
        • et al.
        Hypoxia mediates low cell-cycle activity and increases the proportion of long-term–reconstituting hematopoietic stem cells during in vitro culture.
        Exp Hematol. 2010; 38 (e2): 301-310
        • Anthony B.A.
        • Link D.C.
        Regulation of hematopoietic stem cells by bone marrow stromal cells.
        Trends Immunol. 2014; 35: 32-37
        • Méndez-Ferrer S.
        • Michurina T.V.
        • Ferraro F.
        • Mazloom A.R.
        • MacArthur B.D.
        • Lira S.A.
        • et al.
        Mesenchymal and haematopoietic stem cells form a unique bone marrow niche.
        Nature. 2010; 466: 829-834
        • Cheng T.
        • Rodrigues N.
        • Shen H.
        • Yang Y.-g
        • Dombkowski D.
        • Sykes M.
        • et al.
        Hematopoietic stem cell quiescence maintained by p21cip1/waf1.
        Science. 2000; 287: 1804-1808
        • Nombela-Arrieta C.
        • Pivarnik G.
        • Winkel B.
        • Canty K.J.
        • Harley B.
        • Mahoney J.E.
        • et al.
        Quantitative imaging of haematopoietic stem and progenitor cell localization and hypoxic status in the bone marrow microenvironment.
        Nat Cell Biol. 2013; 15: 533-543
      1. Andrews RG, Bryant EM, Bartelmez SH, Muirhead DY, Knitter GH, Bensinger W., et al. CD34+ marrow cells, devoid of T and B lymphocytes, reconstitute stable lymphopoiesis and myelopoiesis in lethally irradiated allogeneic baboons. 1992.

        • Asl K.D.
        • Shafaei H.
        • Rad J.S.
        • Nozad H.O.
        Comparison of characteristics of human amniotic membrane and human adipose tissue derived mesenchymal stem cells.
        World J Plast Surg. 2017; 6: 33
        • Mazloumi Z.
        • Farahzadi R.
        • Rafat A.
        • Asl K.D.
        • Karimipour M.
        • Montazer M.
        • et al.
        Effect of aberrant DNA methylation on cancer stem cell properties.
        Exp Mol Pathol. 2022; 104757
        • Rafat A.
        • Asl K.D.
        • Mazloumi Z.
        • Movassaghpour A.A.
        • Talebi M.
        • Shanehbandi D.
        • et al.
        Telomerase inhibition on acute myeloid leukemia stem cell induced apoptosis with both intrinsic and extrinsic pathways.
        Life Sci. 2022; 295120402
        • Calvi L.
        • Adams G.
        • Weibrecht K.
        • Weber J.
        • Olson D.
        • Knight M.
        • et al.
        Osteoblastic cells regulate the haematopoietic stem cell niche.
        Nature. 2003; 425: 841-846
        • Wu A.
        • Till J.
        • Siminovitch L.
        • McCulloch E.
        A cytological study of the capacity for differentiation of normal hemopoietic colony‐forming cells.
        J Cell Physiol. 1967; 69: 177-184
        • Nygren J.M.
        • Bryder D.
        • Jacobsen S.E.W.
        Prolonged cell cycle transit is a defining and developmentally conserved hemopoietic stem cell property.
        J Immunol. 2006; 177: 201-208
        • Hock H.
        • Hamblen M.J.
        • Rooke H.M.
        • Schindler J.W.
        • Saleque S.
        • Fujiwara Y.
        • et al.
        Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells.
        Nature. 2004; 431: 1002-1007
        • Sconocchia G.
        • Provenzano M.
        • Rezvani K.
        • Li J.
        • Melenhorst J.
        • Hensel N.
        • et al.
        CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines.
        J Trans Med. 2005; 3: 15
        • Luevano M.
        • Madrigal A.
        • Saudemont A.
        Generation of natural killer cells from hematopoietic stem cells in vitro for immunotherapy.
        Cell Mol Immunol. 2012; 9: 310-320
        • Kao I.-T.
        • Yao C.-L.
        • Kong Z.-L.
        • Wu M.-L.
        • Chuang T.-L.
        • Hwang S.-M.
        Generation of natural killer cells from serum-free, expanded human umbilical cord blood CD34+ cells.
        Stem Cells Dev. 2007; 16: 1043-1052
        • Asl K.D.
        • Rafat A.
        • Movassaghpour A.A.
        • Charoudeh H.N.
        • Nasrabadi H.T.
        • Nasrabadi H.T.
        The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia.
        Adv Pharm Bull. 2021;
        • Whetton A.
        • Dexter T.
        Effect of haematopoietic cell growth factor on intracellular ATP levels.
        Nature. 1983; 303: 629-631
        • Whetton A.D.
        • Bazill G.
        • Dexter T.M.
        Haemopoietic cell growth factor mediates cell survival via its action on glucose transport.
        EMBO J. 1984; 3: 409-413
        • Dexter T.M.
        • Whetton A.D.
        • Bazill G.W.
        Haemopoietic cell growth factor and glucose transport: Its role in cell survival and the relevance of this in normal haemopoiesis and leukaemia.
        Differentiation. 1984; 27: 163-167
        • Rafat A.
        • Dizaji Asl K.
        • Mazloumi Z.
        • Movassaghpour A.A.
        • Farahzadi R.
        • Nejati B.
        • et al.
        Telomerase‐based therapies in haematological malignancies.
        Cell Biochem Funct. 2022;
        • Carmeliet P.
        • Dor Y.
        • Herbert J.-M.
        • Fukumura D.
        • Brusselmans K.
        • Dewerchin M.
        • et al.
        Role of HIF-1α in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.
        Nature. 1998; 394: 485-490
        • Goda N.
        • Ryan H.E.
        • Khadivi B.
        • McNulty W.
        • Rickert R.C.
        • Johnson R.S.
        Hypoxia-inducible factor 1α is essential for cell cycle arrest during hypoxia.
        Mol Cell Biol. 2003; 23: 359-369