Abstract
Background and objectives
In transfusion medicine, the safety of platelet concentrates (PCs) is a major concern
on account of contamination, mostly with Staphylococcus species. One of the most common contaminants is Staphylococcus aureus, which forms bacterial biofilms in PCs, posing a safety risk for transfusion patients.
In this study, we investigate the contributions to biofilm formation of eno, ebps,
and fib genes encoding surface proteins and of genes from the ica operon (icaA and icaD) encoding polysaccharide intercellular adhesin (PIA), along with their expression
in bacteria grown in glucose-supplemented trypticase soy broth (TSBg) and PCs.
Materials and methods
Two strains of S. aureus (2039 and 2110) captured during routine PC screening were tested for biofilm formation
in TSBg and under PC storage conditions, with mRNA collected at five time points and
analyzed to determine expression of eno, ebps, fib, icaA, and icaD and their contributions to biofilm formation in both media.
Results
In TSBg, S. aureus strain 2039 formed weak biofilms while 2110 formed strong. biofilms; however, in
PCs, both strains formed strong biofilms. During biofilm formation, expression levels
of icaA and icaD in both strains were generally significantly higher in TSBg than PCs. In contrast,
expression of eno, ebps, and fib genes tended to be significantly higher under PC
storage conditions.
Conclusion
This study demonstrated that expression of genes involved in biofilm formation can
be affected by growth media. Further investigation is needed to understand biofilm
formation in the PC milieu and enhance transfusion safety.
Keywords
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Article info
Publication history
Published online: May 10, 2022
Accepted:
May 9,
2022
Received in revised form:
May 4,
2022
Received:
March 11,
2022
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
