Abstract
Background
The regenerative potential of platelet lysate (PL) and platelet gel (PG) is mediated
by the release of platelets (PLTs) growth factors. The aim of this study was the evaluation
of the PL production utilizing low volume single Cord Blood Units (CBUs) and the comparison
of the biomolecule content between PLs obtained from intermediate and high volume
CBUs.
Methods
CBUs (n = 90) with volumes greater than 50 ml and initial platelet count > 150 × 109/L were used. CBUs were classified into the following groups: group A (50–80 ml),
group B (81–110 ml) and group C (111–150 ml). The CBUs were centrifuged twice for
the production of the platelet concentrate (PC), which was stored at − 80 °C for at
least 48 h. Then, rapidly thawed and the biomolecule content was determined using
commercial ELISA kits. The regenerative potential of PLs was evaluated using the scratch
wound and in vitro angiogenesis assay.
Results
CBPL was produced from low volume single CBUs and contained 3.4 ± 0.3 ×109 PLTs. PL obtained from intermediate and high volume CBUs consisted of 10.2 ± 0.3
and 16.1 ± 0.4 × 109 PLTs. All PL groups were characterized by high biomolecule content. Gap closure was
observed within 72 h after the wound assay initiation and the capillary tubes were
formed in all study groups.
Conclusion
This study provided significant evidence regarding the utilization of the low volume
CBUs for the production of CB derivatives, thus can serve as healing mediators in
regenerative medicine approaches.
Keywords
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Article info
Publication history
Published online: May 20, 2022
Accepted:
May 17,
2022
Received in revised form:
May 13,
2022
Received:
March 22,
2022
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
