Abstract
Background
Umbilical cord blood (UCB) has improved into an attractive and alternative source
of allogeneic hematopoietic stem cells (all-HSCs) in clinics and, research for three
decades. Recently, it has been shown that the limited cell dose of, this valuable
source can be enhanced by the ex vivo expansion of cells in many, ways. We evaluated
the expression of the Gata transcription factors family and FOG-1, in expanded and
differentiated cord blood-derived CD34 + hematopoietic stem cells to, megakaryocytes
lineage., Methods: Separated mononuclear cells were cultured in DMEM complete medium.,
Harvested cells as a mesenchymal stem cell at 85 % confluency were cultured with,
trypsin/EDTA and in 24-well plates. The characteristic analyses of isolated UCB- MSCs,
were done by flow cytometry and adipogenic, chondrogenic, and osteogenic, differentiation
assays. MACS purified UCB-CD34 + hematopoietic cells cultivated and, differentiated
to megakaryocyte progenitor cells in the presence of cytokine cocktail, with UCB-MSCs.
Then, the GATA1, GATA2, GATA3, and FOG-1 genes expression, after differentiation to
megakaryocyte progenitor cells were performed by quantitative, real-time polymerase
chain reaction (PCR)., Results: In this study, the results of real-time-PCR showed
that the fold change, expression of GATA-1, FOG-1, and GATA-2 genes after co-culturing
with UCB-MSCs, significantly increased to 7.3, 4.7, and 3.3-fold in comparison with
control groups;respectively., Conclusion: UCB-MSCs can increase the expansion and
differentiation of UCBCD34 + , to megakaryocyte progenitor cells through upregulation
of GATA-1, GATA-2, and FOG-1 gene expression.
Keywords
To read this article in full you will need to make a payment
Purchase one-time access:
Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online accessOne-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:
Subscribe to Transfusion and Apheresis ScienceAlready a print subscriber? Claim online access
Already an online subscriber? Sign in
Register: Create an account
Institutional Access: Sign in to ScienceDirect
References
- Factors influencing the umbilical cord blood stem cell industry: an evolving treatment landscape.Stem Cells Transl Med. 2018; 7: 643-650
- The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.Development. 2020; 147dev177022
- Insights and hopes in umbilical cord blood stem cell transplantations.J BioMed Res. 2012; : 2012
- Umbilical cord blood transplantation: the first 25 years and beyond.Blood. 2013; 122: 491-498
- Ex vivo expansion of umbilical cord blood for transplantation.Best Pract Res Clin Hematol. 2010; 23: 245-257
- Paracrine molecules of mesenchymal stem cells for hematopoietic stem cell niche.Bone Marrow Res. 2011; 2011353878
- Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood.Stem Cells. 2004; 22: 625-634
- Isolation and characterization of mesenchymal stromal cells from human umbilical cord and fetal placenta.J Vis Exp. 2017;
- Mesenchymal stem cells from umbilical cord blood: parameters for isolation, characterization, and adipogenic differentiation.Cytotechnology. 2012; 64: 511-521
Asadpoor Dezaki Z., Kheirandish M. Hypoxia Preconditioning Promotes Survival And Clonogenic Capacity Of Human Umbilical Cord Blood Mesenchymal Stem Cells. Iranian Journal of Blood and Cancer.43–49.
- Proliferation extent of CD34+ cells as a key parameter to maximize megakaryocytic differentiation of umbilical cord blood-derived hematopoietic stem/progenitor cells in a two-stage culture protocol.Biotechnol Rep. 2014; 4: 50-55
- A New two step induction protocol for neural differentiation of human umbilical cord blood-derived mesenchymal stem cells.Iran J Blood Cancer. 2015;
- Effects of CXCR1 and CXCR2 inhibition on expansion and differentiation of umbilical cord blood CD133+ cells into megakaryocyte progenitor cells.Cytokine. 2011; 55: 181-187
- The impact of mesenchymal stem cells on differentiation of hematopoietic stem cells.Adv Pharm Bull. 2015; 5: 299-304
- Platelet growth factors suppress ex vivo expansion and enhance differentiation of umbilical cord blood CD133+ stem cells to megakaryocyte progenitor cells.Growth Factors. 2010; 28: 409-416
- Megakaryocytic differentiation induced in 416B myeloid cells by GATA-2 and GATA-3 transgenes or 5-azacytidine is tightly coupled to GATA-1 expression.Blood. 1993; 82: 1493-1501
- Enforced expression of the GATA-3 transcription factor affects cell fate decisions in hematopoiesis.Exp Hematol. 2001; 29: 971-980
- GATA1-mediated megakaryocyte differentiation and growth control can be uncoupled and mapped to different domains in GATA1.Mol Cell Biol. 2005; 25: 8592-8606
- GATA-2 reinforces megakaryocyte development in the absence of GATA-1.Mol Cell Biol. 2009; 29: 5168-5180
- Umbilical cord blood transplantation: Still growing and improving.Stem Cells Transl Med. 2021; 10: S62-S74
- The effect of hypoxia preconditioning on the neural and stemness genes expression profiling in human umbilical cord blood mesenchymal stem cells.Transfus Apher Sci. 2017; 56: 392-399
- Neuroprotective effects of transplanted mesenchymal stromal cells-derived human umbilical cord blood neural progenitor cells in EAE.Iran J Allergy Asthma Immunol. 2015; 14: 596-604
- Improving the neuronal differentiation efficiency of umbilical cord blood-derived mesenchymal stem cells cultivated under appropriate conditions.Iran J Basic Med Sci. 2015; 18: 1100-1106
- Bone marrow and umbilical cord blood human mesenchymal stem cells: state of the art.Int J Clin Exp Med. 2010; 3: 248-269
- Hematopoietic stem-cell transplantation.N Engl J Med. 2006; 354: 1813-1826
- Superior ex vivo cord blood expansion following co-culture with bone marrow-derived mesenchymal stem cells.Bone Marrow Transplant. 2006; 37: 359-366
- Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells.Mol Biosyst. 2010; 6: 1207-1215
- A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells.Exp Hematol. 2005; 33: 828-835
- Mesenchymal stem cells from bone marrow show a stronger stimulating effect on megakaryocyte progenitor expansion than those from non-hematopoietic tissues.Platelets. 2010; 21: 199-210
- Expansion of megakaryocyte precursors and stem cells from umbilical cord blood CD34+ cells in collagen and liquid culture media.J Hematotherapy Stem Cell Res. 2001; 10: 391-403
- Human mesenchymal stem cells support megakaryocyte and pro‐platelet formation from CD34+ hematopoietic progenitor cells.J Cell Physiol. 2000; 184: 58-69
- Combinatorial regulation of tissue specification by GATA and FOG factors.Development. 2012; 139: 3905-3916
- FOG, a multitype zinc finger protein, acts as a co-factor for transcription factor GATA-1 in erythroid and megakaryocytic differentiation.Cell. 1997; 90: 109-119
- Overexpression of GATA-2 inhibits erythroid and promotes megakaryocyte differentiation.Exp Hematol. 2000; 28: 1423-1431
Article info
Publication history
Published online: June 06, 2022
Accepted:
June 3,
2022
Received in revised form:
May 26,
2022
Received:
November 29,
2021
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
