Abstract
Background and objectives
DEL (D-elute) red blood cells (RBCs) are typed as D- by routine serological methods, as
they carry a very weak form of D variant. Asia type DEL (c.1227 G>A) is the most prevalent
DEL allele in East Asian populations that can lead to alloimmunization in D negative
transfusion recipients. This study aimed to screen D+ and D- Thai blood donors for
the Asia type DEL and its zygosity.
Materials and methods
Blood samples of 723 D+ , and 191 D- Thai blood donors were collected. D antigen was
typed by the routine serological method and adsorption-elution test to screen DEL
phenotype. The hybrid Rhesus box, RHD 1227 G, RHD 1227 A and RHD exon 4 were analyzed with polymerase chain reaction using sequence specific primers,
PCR-SSP.
Results
Among 191 D- blood donors, 52 (27.2%) RHD 1227 A allele carriers were detected, and 39 out of these 52 (75.0%) were positive
for the hybrid Rhesus box (i.e. hemizygous RHD 1227 A). The remaining carriers were RHD 1227 A homozygous. RHD zygosity analysis in Thai D+ blood donors showed that only 4% (29/723) had a Dd genotype (hemizygous RHD 1227 G). Five (0.69%) blood donors were detected with RHD 1227 A alleles among 723 D+ blood donors. They were all G/A heterozygous at the RHD 1227 site.
Conclusion
This study indicates that the high prevalence of RHD 1227 A allele among serologically apparent D- blood donors in Thais. Furthermore,
the screening of hybrid Rhesus box, RHD 1227 A combined with RHD exon 4 is useful for analyses of Asia type DEL and its zygosity.
Keywords
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Article Info
Publication History
Published online: June 25, 2022
Accepted:
June 24,
2022
Received in revised form:
April 18,
2022
Received:
November 21,
2021
Identification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
