Cryopreservation of apheresis platelets treated with riboflavin and UV light

Published:September 19, 2022DOI:



      Pathogen reduction technology (PRT) is increasingly used in the preparation of platelets for therapeutic transfusion. As the Czech Republic considers PRT, we asked what effects PRT may have on the recovery and function of platelets after cryopreservation (CP), which we use in both military and civilian blood settings.

      Study design and methods

      16 Group O apheresis platelets units were treated with PRT (Mirasol, Terumo BCT, USA) before freezing; 15 similarly collected units were frozen without PRT as controls. All units were processed with 5–6% DMSO, frozen at − 80 °C, stored > 14 days, and reconstituted in thawed AB plasma. After reconstitution, all units were assessed for: platelet count, mean platelet volume (MPV), platelet recovery, thromboelastography, thrombin generation time, endogenous thrombin potential (ETP), glucose, lactate, pH, pO2, pCO2, HCO3, CD41, CD42b, CD62, Annexin V, CCL5, CD62P, and aggregates > 2 mm and selected units for Kunicki score.


      PRT treated platelet units had lower platelet number (247 vs 278 ×109/U), reduced thromboelastographic MA (38 vs 62 mm) and demonstrated aggregates compared to untreated platelets. Plasma coagulation functions were largely unchanged.


      Samples from PRT units showed reduced platelet number, reduced function greater than the reduced number would cause, and aggregates. While the platelet numbers are sufficient to meet the European standard, marked platelets activation with weak clot strength suggest reduced effectiveness.


      α angle (clot growth), C-CPs (untreated Control-cryopreserved platelet(s)), C-APs (untreated Control-fresh apheresis platelet(s)), CPs (cryopreserved platelet(s)), DMSO (dimethylsulfoxyide), ETP (endogenous thrombin potential), FACs (Flow cytometry), MA (maximum amplitude), MPV (mean platelet volume), PLTs (platelet(s)), PRT (pathogen reduction technology), Peak (maximum amount of thrombin formed), tPeak (maximal thrombin concentration in the sample), R (speed of cloth formation), SE (secondary electron), T-CPs (Treated cryopreserved platelet(s)), TEG (thromboelastography), T-APs (Fresh apheresis platelet(s) before PRT), T-PRAPs (Treated-fresh apheresis platelet(s)), TGT (thrombin generation time), tLag (lag-time before the beginning of accelerated thrombin production)


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