Abstract
Introduction
The isolation of microparticles (MPs) from leukoreduction filters (LRFs) during cell
extraction process introduced LRFs as a precious source of MPs for animal and human
study.
Method
LRFs were collected from Tehran Blood Transfusion Center. The back-flushing method
was used for leukocyte extraction from the LRFs. MPs were isolated through double-step
centrifugation. Dynamic light scattering (DLS), electron microscopy (EM), and flow
cytometry were performed for the evaluation of MPs size, morphology, and structural
properties respectively. Statistical analyses were carried out to evaluation of differences
between test and control groups. a p-value less than 0.05 indicates significant differences.
Result
DLS analysis showed that the average MP size in the test and control groups was 654.83 nm
and 233.68 nm respectively. SEM images showed the spherical, oval, cell fragment,
and micro-aggregate particles and TEM images demonstrated the mitochondrial-like body
in the MPs. Flow cytometry studies also showed a significant increase in the percent
of CD41, and CD14, and a significant decrease in the percent of CD235a in the test
group compared to control (P value=0.029, P value=0.035, P value= 0.001 respectively).
Moreover, the percentage of CD34 MPs indicated a borderline difference between the
two groups (P value= 0.075). Finally count of MPs in the test and control groups was
1202095.34 and 280948.64, respectively and the difference was significant (P value=0.008).
Conclusion
It is concluded that LRFs are a potential source of the large volume of various cell
MPs with different phenotypical and structural properties for animal and human phase
studies. Moreover, the investigation of LRFs as a source of different types of exosomes
can shed new light on extracellular vesicle studies.
Keywords
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Article info
Publication history
Published online: November 03, 2022
Accepted:
November 2,
2022
Received in revised form:
September 22,
2022
Received:
April 22,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2022 Elsevier Ltd. All rights reserved.
