Abstract
Background
Massive hemorrhage is a leading cause of death from trauma. There is growing interest
in group O whole blood transfusions to mitigate coagulopathy and hemorrhagic shock.
Insufficient availability of low-titer group O whole blood is a barrier to routine
use. We tested the efficacy of the Glycosorb® ABO immunoadsorption column to reduce
anti-A/B titers in group O whole blood.
Methods
Six group O whole blood units were collected from healthy volunteers, and centrifuged
to separate platelet poor plasma. Platelet-poor plasma was filtered through a Glycosorb®
ABO antibody immunoabsorption column, then reconstituted to prepare post-filtration
whole blood. Anti-A/B titers, CBC, free hemoglobin, and thromboelastography (TEG)
assays were performed on pre-and post-filtration whole blood.
Results
Mean( ± SEM) anti-A (224 ± 65 pre vs 13 ± 4 post) and anti-B (138 ± 38 pre vs 11 ± 4
post) titers were significantly reduced (p = 0.004) in post-filtration whole blood.
No significant changes were detected in CBC, free hemoglobin, and TEG parameters on
day 0. Free hemoglobin increased throughout storage (48 mg/dl ± 24 Day 0 vs 73 ± 35
Day 7 vs 96 ± 44 Day 14; p = 0.14).
Conclusions
The Glycosorb® ABO column can significantly reduce anti-A/B isoagglutinin titers of
group O whole blood units. Glycosorb® ABO could be employed to provide whole blood
with lower risk of hemolysis and other consequences of infusing ABO incompatible plasma.
Preparation of group O whole blood with substantially reduced anti-A/B would also
increase the supply of low-titer group O whole blood for transfusion.
Keywords
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Article info
Publication history
Published online: March 03, 2023
Accepted:
February 27,
2023
Received in revised form:
February 17,
2023
Received:
November 10,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2023 Elsevier Ltd. All rights reserved.
